CST, 12124S, WIPI1 Antibody

Polyclonal Antibody for studying WIPI1. Validated for Western Blotting. Highly specific and rigorously validated in-house, WIPI1 Antibody (CST #12124) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity WIPI1 Antibody recognizes endogenous levels of total WIPI1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys369 of human WIPI1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. Vacuolar trafficking and autophagy are controlled by the class III type phosphoinositide 3-kinase (PI3K) Vps34, which generates phosphoinositide-3-phosphate (PtdIns3P) (4,5). Atg18 and Atg21 are two related WD-repeat proteins that bind PtdIns3P via a conserved Phe-Arg-Arg-Gly motif (6,7). It has been shown that Atg18 binds to Atg2 and that this complex is directed to vacuolar membranes by its interaction with PtdIns3P (8). Human orthologs of Atg18 and Atg21 were identified as members of the WD-repeat protein interacting with phosphoinositides (WIPI) family (9-11). WIPI1 (also called WIPI49) and WIPI2 have been shown to translocate from several vacuolar compartments to LC3-positive autophagosomes during autophagy; this translocation may be used as an autophagy marker (10). Alternate Names ATG18; Atg18 protein homolog; ATG18A; FLJ10055; WD repeat domain phosphoinositide-interacting protein 1; WD repeat domain, phosphoinositide interacting 1; WD40 repeat protein interacting with phosphoinositides of 49 kDa; WD40 repeat protein Interacting with phosphoInositides of 49kDa; WIPI 49 kDa; WIPI-1; WIPI-1 alpha; WIPI1; WIPI49 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48 SOURCE: Rabbit