CST, 12140S, CP110 Antibody

Polyclonal Antibody for studying CP110. Validated for Western Blotting. Highly specific and rigorously validated in-house, CP110 Antibody (CST #12140) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CP110 Antibody recognizes endogenous levels of total CP110 protein. This antibody cross-reacts with a protein of unknown origin at ~65 kDa. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser580 of human CP110 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The centrosome is an organelle that plays an important role in the mammalian cell cycle. Prior to the onset of mitosis, the single interphase centrosome duplicates only once, creating a pair of daughter centrosomes that will form the two spindle poles after breakdown of the nuclear envelope. Failure to duplicate or overduplication of the centrosome can result in polyploidy and genomic instability (reviewed in 1). Centrosomal protein of 110 kDa (CP110) is a cyclin-dependent kinase (CDK) substrate that plays a critical role in promoting the duplication of centrosomes and correct spindle formation (2). In addition, CP110 has been shown to interact with calmodulin (CaM) and centrin to regulate the progression through cytokinesis (3), and with Cep97 and Cep290 to regulate the formation of primary cilia (4,5). CP110 expression is induced in G1/S with peak expression during S-phase. Degradation of CP110 is mediated by cyclin F in G2-phase and is required for normal progression into M-phase (6). Alternate Names 9738; CCP110; CE110; centriolar coiled-coil protein 110; centriolar coiled-coil protein 110kDa; Centriolar coiled-coil protein of 110 kDa; centrosomal protein CP110; Centrosomal protein of 110 kDa; CEP110; CP110; CP110 protein; DKFZp781G1416; KIAA0419 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 130 SOURCE: Rabbit