{"product_id":"cst-12153t","title":"CST,  12153T, IL-6 (D3K2N) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IL6. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-6 (D3K2N) Rabbit Monoclonal Antibody (CST #12153) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIL-6 (D3K2N) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-6 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant human IL-6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAcute phase response is induced by interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells (1,2). IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-β, is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak\/Stat cascade and the SHP-2\/Erk MAP kinase cascade (1,3,4). IL-6 also forms a complex with an IL-6Rα splice variant that is non-membrane-associated (3). The IL-6\/soluble IL-6Rα complex can then activate the gp130 signaling pathway in cells that express gp130 but not IL-6Rα (3). Research studies have shown that IL-6, through increasing expression of proangiogenic VEGF, may also contribute to metastatic breast cancer (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB cell stimulatory factor-2; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; BSF2; CDF; CTL differentiation factor; HGF; HSF; Hybridoma growth factor; IFN-beta-2; IFNB2; IL-6; IL6; Interferon beta-2; interleukin 6; interleukin 6 (interferon, beta 2); interleukin BSF-2; Interleukin-6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 21-28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797566771369,"sku":"12153T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12153t","provider":"Iright","version":"1.0","type":"link"}