CST, 12165S, HSP60 (D6F1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HSP60. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, HSP60 (D6F1) Rabbit Monoclonal Antibody (CST #12165) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56658 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HSP60 (D6F1) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSP60 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Xenopus, Zebrafish, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp68 of human HSP60 protein. Background In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8). Alternate Names 60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; CH60; Chaperonin 60; CPN60; epididymis secretory sperm binding protein; GROEL; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; heat shock protein family D (Hsp60) member 1; HLD4; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13 Specification REACTIVITY: H M R Hm Mk X Z B Pg SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG