{"product_id":"cst-12166t","title":"CST,  12166T, MAML1 (D3K7B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MAML1. Validated for WB,IP,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, MAML1 (D3K7B) Rabbit Monoclonal Antibody (CST #12166) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nChromatin IP: 1:100\nChromatin IP-seq: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMAML1 (D3K7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAML1 protein. This antibody does not detect MAML2 or MAML3.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp269 of human MAML1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMastermind-like (MAML) family of proteins are homologs of Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ53540; KIAA0200; Mam-1; Mam1; MAML1; mastermind homolog; mastermind like transcriptional coactivator 1; mastermind-like 1; mastermind-like 1 (Drosophila); Mastermind-like protein 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 130\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797567262889,"sku":"12166T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12166t","provider":"Iright","version":"1.0","type":"link"}