CST, 12191S, SPT16 (D7I2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SUPT16H/SPT16. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SPT16 (D7I2K) Rabbit Monoclonal Antibody (CST #12191) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity SPT16 (D7I2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPT16 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu662 of human SPT16 protein. Background Suppressor of Ty-16 (SPT16) and structure-specific recognition protein-1 (SSRP1) are subunits of the facilitates chromatin transcription (FACT) complex that is essential for transcription elongation (1,2). FACT facilitates RNA polymerase-dependent transcription of chromatin templates by destabilizing the nucleosomes within the open reading frames of active genes (3-5). FACT destabilizes the nucleosomes, which would otherwise act as barriers to RNA polymerase transcription activity, by disrupting histone-histone and histone-DNA contacts that lead to the eviction of the histone H2A-H2B dimer (2,3,6). FACT may also function as a histone chaperone to reassemble nucleosomes after RNA polymerase passage (7). In addition to transcription, FACT activity has been shown to have a role in DNA replication in yeast and in DNA repair by contributing to the activation of p53 by CK2 and by facilitating histone H2AX-H2B exchange upon DNA damage (8,9). Alternate Names CDC68; Chromatin-specific transcription elongation factor 140 kDa subunit; facilitates chromatin remodeling 140 kDa subunit; Facilitates chromatin transcription complex subunit SPT16; FACT 140 kDa subunit; FACT complex subunit SPT16; FACT140; FACTP140; FLJ10857; FLJ14010; FLJ34357; hSPT16; SP16H; SPT16; SPT16 homolog, facilitates chromatin remodeling subunit; SPT16/CDC68; suppressor of Ty 16 homolog; suppressor of Ty 16 homolog (S. cerevisiae); SUPT16H Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG