CST, 12199S, CCR2 (D14H7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CCR2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CCR2 (D14H7) Rabbit Monoclonal Antibody (CST #12199) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CCR2 (D14H7) Rabbit mAb recognizes endogenous levels of total CCR2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly197 of human CCR2 protein. Background CCR2 is a member of the "CC-branch" of chemokine G protein-coupled receptors that regulate monocyte chemotaxis and T cell migration/activation and drive inflammation in a number of pathological conditions (1). CCR2 is the receptor for several chemokines including MCP-1, MCP-3, and MCP-4 (2-5). CCR2 transduces signals through increases in intracellular calcium levels. It has two alternative isoforms, CCR2A and CCR2B, differing in their carboxy-terminal tails with CCR2B trafficking more efficiently to the membrane (2,6). CCR2 was originally identified in the THP-1 monocyte cell line, and its expression is decreased following differentiation into macrophages (7). Knockout studies demonstrate that CCR2 is a major regulator of macrophage trafficking (8-10). In addition, research studies have shown that CCR2 functions as an alternative coreceptor with CD4 for infection of some strains of HIV (11,12). Alternate Names C-C chemokine receptor type 2; C-C CKR-2; C-C motif chemokine receptor 2; CC-CKR-2; CCR-2; CCR2; CCR2A; CCR2B; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CKR2B; CMKBR2; FLJ78302; MCP-1 receptor; MCP-1-R; MGC103828; MGC111760; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG