CST, 12214T, Rab3A (D7B10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rab3A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Rab3A (D7B10) Rabbit Monoclonal Antibody (CST #12214) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rab3A (D7B10) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab3A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg149 of human Rab3A protein. Background The Rab family of proteins includes small, monomeric GTPases essential for regulating intracellular vesicle trafficking. Members of the Rab3 subfamily, including Rab3A-3D, are involved in the exocytosis of neurotransmitters and hormones (1). Rab3A is primarily expressed in neurons (2), neuroendocrine cells (such as rat PC-12 cells), and in human pancreatic β cells (3,4). By acting as a molecular switch between active GTP-bound Rab3A and the inactive GDP-bound form, Rab3A inhibits synaptic vesicle and chromaffin granule secretion during late membrane release (5,6). Loss-of-function studies suggest Rab3A is involved in controlling synaptic vesicle targeting and docking at the active zone (7). Through binding to its direct effector Rabphillin, Rab3A also orchestrates the coupling between synaptic vesicle exocytosis and endocytosis (8). Alternate Names RAB3A; RAB3A, member RAS oncogene family; RAS-associated protein RAB3A; Ras-related protein Rab-3A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG