{"product_id":"cst-12245t","title":"CST,  12245T, TPX2 (D2R5C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TPX2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, TPX2 (D2R5C) Rabbit Monoclonal Antibody (CST #12245) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTPX2 (D2R5C) Rabbit Monoclonal Antibody recognizes endogenous levels of total TPX2 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys420 of human TPX2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). TPX2, a target of active Ran (RanGTP), is a microtubule nucleating protein. TPX2 is inactive when bound to nuclear importin-alpha. RanGTP activity disrupts this interaction, relieving inhibition of TPX2 (3). TPX2 binding activates Aurora A kinase and promotes its localization to the mitotic spindle (4,5). DNA damage in mitosis leads to loss of interaction between Aurora A and TPX2 and inactivation of Aurora A kinase (6). TPX2 is highly expressed in pancreatic cancer cells, and knockdown of TPX2 expression in these cells is associated with increased sensitivity to paclitaxel (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC20orf1; C20orf2; Differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; p100; preferentially expressed in colorectal cancer; Protein fls353; REPP86; restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; Targeting protein for Xklp2; TPX2; TPX2 microtubule nucleation factor; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog; TPX2, microtubule-associated, homolog (Xenopus laevis)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797568278697,"sku":"12245T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12245t","provider":"Iright","version":"1.0","type":"link"}