CST, 12252T, CPT1A (D3B3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CPT1a. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CPT1A (D3B3) Rabbit Monoclonal Antibody (CST #12252) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CPT1A (D3B3) Rabbit Monoclonal Antibody recognizes endogenous levels of total CPT1A protein. This antibody recognizes endogenous levels of total CPT1A protein. This antibody does not cross-react with CPT1B, CPT1C, or CPT2. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu213 of human CPT1A protein. Background Carnitine palmitoyltransferase-1 (CPT1), localized to the mitochondrial outer membrane, translocates fatty acids across the mitochondrial membranes and catalyzes the rate-limiting step of β-oxidation (1,2). There are three isoforms of this enzyme: CPT1A (liver), CPT1B (muscle), and CPT1C (brain) (1,2). Deficiency of CPT1A results in an autosomal recessive mitochondrial fatty acid oxidation disorder (3). Studies have shown that physiological high blood glucose and insulin levels inhibit CPT1B activity in human muscle and therefore divert long-chain fatty acids toward storage in human muscle as triglycerides (4). Furthermore, mice deficient in CPT1C show less food intake and reduced body weight (5). These findings suggest that CPT1 may play a role in metabolic syndromes. Alternate Names Carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase 1A (liver); carnitine palmitoyltransferase I, liver; CPT I; CPT1; CPT1-L; CPT1A; CPTI-L; L-CPT1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 88 Source/Isotype: Rabbit IgG