CST, 12254T, Plectin-1 (D6A11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying plectin. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Plectin-1 (D6A11) Rabbit Monoclonal Antibody (CST #12254) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Plectin-1 (D6A11) Rabbit Monoclonal Antibody recognizes endogenous levels of total plectin-1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu2980 of human plectin-1 protein. Background Plectin is a large, widely expressed protein that crosslinks the intermediate filament and actin cytoskeleton, mechanically stabilizing cells and tissues. Plectin also plays a role in the regulation of actin dynamics and acts as a scaffold for signaling molecules (1). Plectin is important in the stabilization of hemidesmosomes, crosslinking them to the intermediate filament network. Research studies have shown that mutations in plectin and other genes coding for hemidesmosomal proteins can cause epidermolysis bullosa, a condition manifested by fragile skin and frequent blistering (1,2). Plectin modulates signals to PKC through binding and sequestration of RACK1, the receptor for activated C kinase 1 (3,4). Plectin is also involved in the regulation of cytokeratin architecture and cell stress response (4), signaling through the chemokine receptor CXCR4 (5) and regulation of AMP-activated protein kinase (AMPK) activity and signaling in mouse myotubes (6). Alternate Names EBS1; EBSMD; EBSND; EBSO; EBSOG; EBSPA; HD1; Hemidesmosomal protein 1; LGMD2Q; LGMDR17; PCN; PLEC; PLEC1; PLEC1b; Plectin; plectin 1, intermediate filament binding protein 500kDa; Plectin-1; PLTN Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 400-500 Source/Isotype: Rabbit IgG