CST, 12258S, E2A (D2B1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying E2A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, E2A (D2B1) Rabbit Monoclonal Antibody (CST #12258) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity E2A (D2B1) Rabbit Monoclonal Antibody recognizes endogenous levels of total E2A protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly112 of human E2A protein. Background E2A is a member of the E-protein family of transcription factors, a subclass of basic helix-loop-helix (bHLH) proteins that bind specifically to E-box consensus sequences (1,2). Alternative splicing generates two E2A isoforms (E47 and E12) that are actively involved in B cell lineage commitment, B cell maturation, IgK V-J rearrangement, peripheral B cell development, and tumor suppression (3). E2A acts in during G1 to promote immunoglobulin gene diversification (4). Research studies have shown that chromosomal translocations involving the E2A gene result in the expression of multiple fusion proteins and are associated with many cases of pediatric acute lymphoblastic leukemia (5). Alternate Names AGM8; BHLHB21; Class B basic helix-loop-helix protein 21; E2A; E2A immunoglobulin enhancer-binding factor E12/E47; E47; helix-loop-helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; Kappa-E2-binding factor; MGC129647; MGC129648; negative vitamin D response element-binding protein; NOL1-TCF3 fusion; p75; TCF-3; TCF3; TFE2; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); transcription factor 3 variant 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDIR; VDR interacting repressor; vitamin D receptor-interacting repressor Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG