CST, 12271S, beta1-Adrenergic Receptor Antibody

Polyclonal Antibody for studying adrenergic receptor beta-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, beta1-Adrenergic Receptor Antibody (CST #12271) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity beta1-Adrenergic Receptor Antibody recognizes transfected levels of total human β1-Adrenergic Receptor protein. This antibody does not cross-react with β2-Adrenergic Receptor. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly406 of human β1-Adrenergic Receptor protein. Antibodies are purified by protein A and peptide affinity chromatography. Background β1-Adrenergic Receptor (β1AR) is a G protein-coupled receptor (GPCR) involved in the regulation of cardiovascular functions (1). Together with β2AR, β1AR is a major βAR in the heart. β1AR is activated by catecholamines and couples to Gα protein, activating adenylate cyclase and increasing intracellular cAMP levels (2). Beta-blockers (βAR antagonists), one of the major class of therapeutics in cardiovascular medicine, act mostly by preventing catecholamine binding to β1AR (3). Alternate Names ADRB1; ADRB1R; adrenergic, beta-1-, receptor; adrenoceptor beta 1; B1AR; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; BETA1AR; FNSS2; RHR Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 50-200 SOURCE: Rabbit