{"product_id":"cst-12292t","title":"CST,  12292T, TEAD1 (D9X2L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TEAD1. Validated for WB,WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, TEAD1 (D9X2L) Rabbit Monoclonal Antibody (CST #12292) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 95633 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTEAD1 (D9X2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total TEAD1 protein.\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe193 of human TEAD1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned on, YAP is phosphorylated by LATS1\/2 kinase and sequestered in the cytoplasm by 14-3-3 protein binding, rendering YAP inactive. When the Hippo pathway is off, non-phosphorylated YAP translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP\/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAA; atrophia areata, peripapillary chorioretinal degeneration; NTEF-1; Protein GT-IIC; REF1; SV40 transcriptional enhancer factor; TCF-13; TCF13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEA domain transcription factor 1; TEAD-1; TEAD1; TEF-1; TEF1; Transcription factor 13; transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797569556649,"sku":"12292T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12292t","provider":"Iright","version":"1.0","type":"link"}