{"product_id":"cst-12301s","title":"CST,  12301S, APC2 Antibody","description":"Polyclonal Antibody for studying APC2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, APC2 Antibody (CST #12301) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAPC2 Antibody recognizes endogenous levels of total APC2 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys458 of human APC2 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex\/cyclosome (APC\/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC\/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC\/C subunits have been identified. Like all E3 enzymes, APC\/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed, APC\/C has been shown to interact with UBE2S and UBE2C E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). APC\/C activity is also strictly dependent upon its association with multiple cofactors. For example, the related proteins, Cdc20 and Cdh1\/FZR1, participate in the recognition of APC\/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8). Anaphase-promoting complex subunit 2 (APC2) is a distant member of the cullin family (9,10) that interacts with a RING-H2 finger protein related to Rbx1\/Hrt1\/Roc1, called APC11, to form the catalytic subcomplex of the APC\/C. The APC2\/11 subcomplex recruits E2 enzymes such as UBE2C\/UBCH10 and is required for the APC\/C to catalyze substrate ubiquitination (11). Therefore, APC is a member of the expanding family of cullin-RING finger-based ubiquitin ligases. The physiologic importance of APC2 was underscored by the finding that disruption of murine causes embryonic lethality (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nANAPC2; anaphase promoting complex subunit 2; Anaphase-promoting complex subunit 2; ANC2; APC2; Cyclosome subunit 2; KIAA1406; RP11-350O14.5\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797569851561,"sku":"12301S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12301s","provider":"Iright","version":"1.0","type":"link"}