CST, 12304T, CIN85 (D1A4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CIN85. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CIN85 (D1A4) Rabbit Monoclonal Antibody (CST #12304) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CIN85 (D1A4) Rabbit Monoclonal Antibody recognizes endogenous levels of total CIN85 protein. This antibody also detects CIN85 isoform b (CD2BP3) and CIN85 isoform c. This antibody does not cross-react with CMS/CD2AP. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys485 of human CIN85 protein. Background CIN85 was independently identified as Cbl-interacting protein of 85 kDa (1), Ruk (regulator of ubiquitous kinase) (2), SETA (SH3 domain-containing gene expressed in tumorigenic astrocytes) (3), and SH3KBP1 (SH3 domain kinase binding protein 1) (4). The genes encoding these proteins were isolated from either human (CIN85), rat (Ruk and SETA), or mouse (SH3KBP1) sources and share between 92% and 97% sequence identity, suggesting that they represent homologues of one gene. Differential promoter usage and alternative splicing is thought to occur in a tissue specific and developmentally regulated manner to generate a complex expression pattern of various transcripts and encoded protein isoforms (5). The main isoform in humans, CIN85, contains three N-terminal SH3 domains, a proline-rich region harboring several P-X-X-P motifs that provide recognition sites for SH3 domain-containing proteins, a PEST sequence implicated in CIN85 degradation, and a C-terminal coiled-coil region for oligomerization (1,2,5,6). The other molecular variants of CIN85 are shorter, N-terminally truncated proteins lacking one, two, or all three of the SH3 domains (1,5,6-8). Proteomic screens suggest that CIN85 is phosphorylated at multiple sites and the role of phosphorylation of some of these sites in regulation of intra- and intermolecular interactions of CIN85 cannot be excluded. CIN85 belongs to the CD2AP/CMS family of adaptor proteins and has been shown to interact with signaling molecules such as c-Cbl, Cbl-b, BLNK, p85/PI3K, GRB2, p130 Cas, and endophilins to coordinate the activity of multiple signaling cascades. Indeed, a growing body of evidence suggests that CIN85 is required for the regulation of a variety of cellular processes including vesicle-mediated transport (9-12), signal transduction (13,14), and cytoskeleton remodelling (15). Alternate Names AGMX2; c-Cbl-interacting protein; Cbl-interacting protein of 85 kDa; CD2-binding protein 3; CD2BP3; CIN85; GIG10; HSB-1; HSB1; Human Src family kinase-binding protein 1; IMD61; MIG18; migration-inducing gene 18; migration-inducing gene 18 protein; SH3 domain containing kinase binding protein 1; SH3 domain-containing kinase-binding protein 1; SH3-domain kinase binding protein 1; SH3K1; SH3KBP1; Src family kinase-binding protein 1; src-related kinase binding protein-1 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 78, 82 Source/Isotype: Rabbit IgG