CST, 12305S, Thap11/Ronin Antibody

Polyclonal Antibody for studying THAP11. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Thap11/Ronin Antibody (CST #12305) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Thap11/Ronin Antibody recognizes endogenous levels of total Thap11/Ronin protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Thap11 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Thanatos-associated protein (Thap) proteins are a family of cellular factors that are characterized by an evolutionarily conserved protein motif similar to the DNA-binding domain of P element transposase (1). There are 12 known human Thap proteins that all act as site-specific DNA-binding factors involved in transcriptional regulation, cell proliferation, chromatin modification, and apoptosis (2-4). Human Thap11 has been shown to suppress cell growth through transcriptional suppression of c-Myc (5). The mouse homolog of Thap11, Ronin, has been identified as an essential factor underlying embryogenesis in mouse embryonic stem cells (6). Alternate Names CTG-B43a; CTG-B45d; HRIHFB2206; RONIN; THA11; THAP domain containing 11; THAP domain-containing protein 11; THAP11 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 49 SOURCE: Rabbit