CST, 12316S, Phospho-Scribble (Ser1220) (D8A2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Scribble (Ser1220) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Scribble (Ser1220) (D8A2) Rabbit Monoclonal Antibody (CST #12316) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Scribble (Ser1220) (D8A2) Rabbit Monoclonal Antibody recognizes endogenous levels of scribble protein only when phosphorylated at Ser1220. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1220 of human scribble protein. Background Scribble (Scrib) was originally identified in a genetic screen in along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds β-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8). The phosphorylation state of Scribble has been shown to be functionally important, in part by regulating subcellular localization (9). Mass spectrometry studies have identified phosphorylation at Ser1220 as a frequent modification in a variety of cell and tissue types (10-13). The functional significance of this modification remains to be elucidated. Alternate Names CRIB1; hScrib; KIAA0147; LAP4; Protein LAP4; Protein scribble homolog; SCRB1; SCRIB; SCRIB1; Scribble; scribble planar cell polarity protein; scribbled homolog; scribbled homolog (Drosophila); scribbled planar cell polarity protein; VARTUL Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 240 Source/Isotype: Rabbit IgG