CST, 12322S, Pumilio 1 Antibody

Polyclonal Antibody for studying PUM1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Pumilio 1 Antibody (CST #12322) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Pumilio 1 Antibody recognizes endogenous levels of total pumilio 1 protein. Species Reactivity: Human, Mouse, All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly169 of human pumilio 1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Pumilio 1 and Pumilio 2 (PUM1, PUM2, or Pumilio homolog 1 and 2, respectively) are evolutionarily conserved RNA binding proteins that are thought to repress translation and stability of mRNA targets by binding to the 3'-UTR of specific RNA sequences (1). Pumilio proteins have been implicated in the regulation of genes involved in embryogenesis and germline cell development (2). Research studies have shown that PUM2 may have a role in neural stem cell fate decisions (3). Alternate Names HsPUM; KIAA0099; PUM1; PUMH; PUMH1; Pumilio homolog 1; pumilio homolog 1 (Drosophila); pumilio RNA binding family member 1; Pumilio-1; PUML1; SCA47 Specification REACTIVITY: H M All SENSITIVITY: Endogenous MW (kDa): 140 SOURCE: Rabbit