CST, 12323S, Pumilio 2 Antibody

Polyclonal Antibody for studying pumillio 2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Pumilio 2 Antibody (CST #12323) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Pumilio 2 Antibody recognizes endogenous levels of total Pumilio 2 protein. This antibody is expected to recognize all three known Pumilio 2 protein isoforms. Cross reactive bands of unknown origin are observed around 45 kDa. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Pumilio 2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Pumilio 1 and Pumilio 2 (PUM1, PUM2, or Pumilio homolog 1 and 2, respectively) are evolutionarily conserved RNA binding proteins that are thought to repress translation and stability of mRNA targets by binding to the 3'-UTR of specific RNA sequences (1). Pumilio proteins have been implicated in the regulation of genes involved in embryogenesis and germline cell development (2). Research studies have shown that PUM2 may have a role in neural stem cell fate decisions (3). Alternate Names FLJ36528; KIAA0235; MGC138251; MGC138253; PUM2; PUMH2; Pumilio homolog 2; pumilio homolog 2 (Drosophila); pumilio RNA binding family member 2; Pumilio-2; PUML2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 110-125 SOURCE: Rabbit