{"product_id":"cst-12354s","title":"CST,  12354S, ARID1A\/BAF250A (D2A8U) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ARID1A. Validated for Western Blotting,Immunohistochemistry (Paraffin),Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, ARID1A\/BAF250A (D2A8U) Rabbit Monoclonal Antibody (CST #12354) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:500 - 1:2000\nChromatin IP: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 87655 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nARID1A\/BAF250A (D2A8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARID1A\/BAF250A protein. This antibody also cross-reacts with proteins of unknown origin at 65 kDa.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1293 of human ARID1A\/BAF250A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI\/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI\/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). ARID1A is one of the accessory subunits of the SWI\/SNF complex that is an essential part of the esBAF (mouse embryonic stem cell specific SWI\/SNF complex). ARID1A is critical for ES cell pluripotency and differentiation into mesoderm-derived cardiomyocytes and adipocytes (10). In addition, ARID1A has been found to be frequently mutated in several cancers such as uterine and ovarian endometrioid carcinoma and ovarian clear cell carcinoma (11-13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nARI1A; ARID domain-containing protein 1A; ARID1A; AT rich interactive domain 1A (SWI- like); AT rich interactive domain 1A (SWI-like); AT-rich interaction domain 1A; AT-rich interactive domain-containing protein 1A; B120; BAF250; BAF250A; BM029; brain protein 120; BRG1-associated factor 250; BRG1-associated factor 250a; C1orf4; chromatin remodeling factor p250; CSS2; ELD; hELD; hOSA1; MRD14; Osa homolog 1; OSA1; OSA1 nuclear protein; P270; SMARCF1; SWI-like protein; SWI\/SNF complex protein p270; SWI\/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1; SWI\/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 270\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797570703529,"sku":"12354S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12354s","provider":"Iright","version":"1.0","type":"link"}