CST, 12439T, CD82 (D7G6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD82. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD82 (D7G6H) Rabbit Monoclonal Antibody (CST #12439) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD82 (D7G6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD82 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile125 of human CD82 protein. Background CD82 (KAI1) belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2). CD82 does not have enzymatic activity and appears to function by regulating the trafficking of other proteins and organization of the cell membrane (1). CD82 was originally described as a costimulator for T cells that directly associates with CD4 and CD8, and was subsequently identified during a screen as a metastasis suppressor in prostate cancer (2,3). CD82 has since been found to act as a metastasis suppressor in a variety of cancers, and its downregulation is associated with poor prognosis in research studies (4-6). CD82 suppresses metastasis through multiple mechanisms including inhibition of cell motility and invasion by modulating c-Met and the urokinase plasminogen activator surface receptor (uPAR), as well as promotion of homotypic cell-cell adhesion by stabilizing interactions between E-cadherin and β-catenin (7-9). Alternate Names 4F9; C33; C33 antigen; CD82; CD82 antigen; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)); CD82 molecule; GR15; IA4; Inducible membrane protein R2; KAI1; kangai 1 (suppression of tumorigenicity 6, prostate; Metastasis suppressor Kangai-1; R2; R2 leukocyte antigen; SAR2; ST6; suppression of tumorigenicity 6; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; Tspan-27; TSPAN27 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-60 Source/Isotype: Rabbit IgG