CST, 12443S, MTMR3 Antibody

Polyclonal Antibody for studying MTMR3. Validated for Western Blotting. Highly specific and rigorously validated in-house, MTMR3 Antibody (CST #12443) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MTMR3 Antibody recognizes endogenous levels of total MTMR3 protein. This antibody also cross-reacts with a protein of unknown origin at 48 kDa. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro600 of human MTMR3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Myotubularin-related proteins are a family of phosphatases with emerging roles in cellular signaling and membrane trafficking (1,2). MTMR3 (Myotubularin-related protein 3), also known as FYVE-DSP1, contains an amino terminal pleckstrin homology (PH) domain and a carboxyl terminal FYVE domain. MTMR3 was first reported as a dual-specific phosphatase, having phosphatase activity toward phosphorylated serine, threonine, and tyrosine residues (3). Subsequent research studies reported that MTMR3 has phosphatase activity toward phosphoinositides, including phosphatadylinositol-3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P ) (4). Accumulation of PI3P by the class III phosphoinositide 3-kinase Vps34 is a key element in autophagosome formation (5). Inhibition of PI3P by MTMR3 can play an important role in suppressing autophagsome formation (6). Alternate Names FLJ32333; FYVE (Fab1 YGLO23 Vsp27 EEA1 domain) dual-specificity protein phosphatase; FYVE domain-containing dual specificity protein phosphatase 1; FYVE-DSP1; KIAA0371; MTMR3; myotubularin related protein 3; Myotubularin-related protein 3; Phosphatidylinositol-3-phosphate phosphatase; Phosphatidylinositol-3,5-bisphosphate 3-phosphatase; ZFYVE10; Zinc finger FYVE domain-containing protein 10; zinc finger, FYVE domain containing 10 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 130-150 SOURCE: Rabbit