CST, 12448S, CK1 epsilon Antibody

Polyclonal Antibody for studying CK1-E. Validated for Western Blotting. Highly specific and rigorously validated in-house, CK1 epsilon Antibody (CST #12448) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CK1 epsilon Antibody recognizes endogenous levels of total CK1ε protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala349 of human CK1ε protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (alpha, alpha prime, beta, gamma 1-3, delta, and epsilon) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1 epsilon (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes, including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of beta-Catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4). CK1ε is involved in many cellular processes, such as differentiation (5-7), cell growth and apoptosis (8), and control of the circadian rhythm (9,10). Alternate Names casein kinase 1 epsilon; casein kinase 1, epsilon; Casein kinase I isoform epsilon; CK1E; CKI-epsilon; CKIe; CKIepsilon; CSNK1E; HCKIE; KC1E; MGC10398 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 43 SOURCE: Rabbit