CST, 12453S, LIS1 Antibody

Polyclonal Antibody for studying LIS1. Validated for Western Blotting. Highly specific and rigorously validated in-house, LIS1 Antibody (CST #12453) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LIS1 Antibody recognizes endogenous levels of total LIS1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly298 of human LIS1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background LIS1 is a cytoskeleton-interacting protein that contains an N-terminal dimerization domain and a C-terminal β-propeller domain that interacts with the motor domain of dynein (1-3). Research studies have shown that mutations in the LIS1 gene are involved in lissencephaly, a disease characterized by severe defects in brain development (4). LIS1 also plays a critical role in cortical migration and development in the brain (5). LIS1 activity is required for retrograde translocation of excitatory synapses in developing interneuron dendrites in a microtubule-dependent fashion (6). Alternate Names LIS-1; LIS1; LIS2; lissencephaly 1 protein; Lissencephaly-1 protein; MDCR; MDS; NudF; PAF acetylhydrolase 45 kDa subunit; PAF-AH 45 kDa subunit; PAF-AH alpha; PAFAH; PAFAH alpha; PAFAH1B1; PAFAHA; platelet activating factor acetylhydrolase 1b regulatory subunit 1; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa); Platelet-activating factor acetylhydrolase IB subunit alpha; Platelet-activating factor acetylhydrolase IB subunit beta; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD); platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 47 SOURCE: Rabbit