CST, 12455T, MacroH2A1.1 (D5F6N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying H2A1.1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MacroH2A1.1 (D5F6N) Rabbit Monoclonal Antibody (CST #12455) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MacroH2A1.1 (D5F6N) Rabbit Monoclonal Antibody recognizes endogenous levels of total MacroH2A1.1 protein. This antibody does not cross-react with other MacroH2A proteins, including MacroH2A1.2 and MacroH2A2. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human MacroH2A1.1 protein. Background Histone macroH2A1 and macroH2A2 comprise a family of variant histone H2A proteins. MacroH2A1 exists as two distinct isoforms due to alternative splicing of a single gene; macroH2A1.1 levels accumulate throughout differentiation and development while macroH2A1.2 shows a constant level of expression (1). MacroH2A1 and macroH2A2 are encoded by completely distinct genes located on separate chromosomes (2,3). Both macroH2A1 and macroH2A2 proteins contain an amino-terminal histone-like region with 64% sequence identity to canonical histone H2A, in addition to a carboxy-terminal "macro" domain (1-3). MacroH2A1 and macroH2A2 are enriched in facultative heterochromatin, including inactivated X chromosomes in mammalian females and senescence-associated heterochromatin foci (2-5). Both act to repress gene transcription by inhibiting the binding of transcription factors to chromatin, the acetylation of histones by p300, and the chromatin-remodeling activities of SWI/SNF and ACF (6,7). The macro domain of macroH2A1.1 binds to ADP-ribose and functions to recruit macroH2A1.1 to activated PARP at sites of DNA damage, where it mediates chromatin rearrangements to locally regulate the DNA damage response (8). MacroH2A1.2 and macroH2A2 do not bind poly-ADP-ribose and are not recruited to sites of activated PARP (8). Alternate Names Core histone macro-H2A.1; H2A histone family member Y; H2A histone family, member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2AFY; H2AY; Histone H2A.y; Histone macroH2A1; histone macroH2A1.1; histone macroH2A1.2; macroH2A.1 histone; MACROH2A1; MACROH2A1.1; macroH2A1.2; Medulloblastoma antigen MU-MB-50.205; mH2A1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG