{"product_id":"cst-12492t","title":"CST,  12492T, DAP12 (D7G1X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Dap12. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DAP12 (D7G1X) Rabbit Monoclonal Antibody (CST #12492) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDAP12 (D7G1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total DAP12 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DAP12 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDNAX-activating protein 12 (DAP12, TYROBP) is a signaling adaptor for several pathogen receptors expressed by cells of the innate immune system (1). The DAP12 protein structure consists of a short extracellular domain, a transmembrane domain, and a cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM) (2). DAP12 protein is expressed by hematopoietic cells, including NK cells, monocytes, macrophages, dendritic cells, mast cells, basophils, eosinophils, neutrophils, and some γδ T cells and NKT cells (1). DAP12 exists as a homodimer that associates with a variety of receptors involved in pathogen detection, including the KIR family of NK cell receptors (2,3). Ligand binding by DAP12-associated receptors results in phosphorylation of tyrosine residues within the DAP12 ITAM by Src family kinases and leads to activation of Syk or Zap-70 and downstream signaling responses (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDAP12; DNAX adaptor protein 12; DNAX-activation protein 12; KAR-associated protein; KARAP; killer activating receptor associated protein; Killer-activating receptor-associated protein; PLOSL; PLOSL1; polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy; transmembrane immune signaling adaptor TYROBP; TYOBP; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein; TYROBP\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 10, 12\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797573259433,"sku":"12492T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12492t","provider":"Iright","version":"1.0","type":"link"}