CST, 12496S, eIF1 (D7G3L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying eIF1. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF1 (D7G3L) Rabbit Monoclonal Antibody (CST #12496) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity eIF1 (D7G3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total eIF1 protein. Based upon sequence alignment, this antibody may cross-react with eIF1B. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human eIF1 protein. Background Eukaryotic translation initiation factor 1 (eIF1) was first purified as a factor stimulating binding of Met-tRNA and mRNA to the ribosome (1,2). eIF1 is essential for growth in yeast and two classes of mutations in yeast eIF1 indicate a role for this protein in ensuring accurate translation initiation site selection (3). It has been demonstrated that eIF1 expression is stress-inducible, suggesting that modulation of translation initiation occurs during cellular stress (4). Alternate Names A121; EIF-1; EIF1; EIF1A; Eukaryotic translation initiation factor 1; ISO1; Protein translation factor SUI1 homolog; SUI1; Sui1iso1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG