{"product_id":"cst-12496s","title":"CST,  12496S, eIF1 (D7G3L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying eIF1. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF1 (D7G3L) Rabbit Monoclonal Antibody (CST #12496) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor more information about the RBP-eCLIP service please visit Eclipsebio .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\neCLIP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\neIF1 (D7G3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total eIF1 protein. Based upon sequence alignment, this antibody may cross-react with eIF1B.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human eIF1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEukaryotic translation initiation factor 1 (eIF1) was first purified as a factor stimulating binding of Met-tRNA and mRNA to the ribosome (1,2). eIF1 is essential for growth in yeast and two classes of mutations in yeast eIF1 indicate a role for this protein in ensuring accurate translation initiation site selection (3). It has been demonstrated that eIF1 expression is stress-inducible, suggesting that modulation of translation initiation occurs during cellular stress (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nA121; EIF-1; EIF1; EIF1A; Eukaryotic translation initiation factor 1; ISO1; Protein translation factor SUI1 homolog; SUI1; Sui1iso1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 13\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797573357737,"sku":"12496S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12496s","provider":"Iright","version":"1.0","type":"link"}