CST, 12503T, Caldesmon-1 (D5C8D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Caldesmon. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Caldesmon-1 (D5C8D) Rabbit Monoclonal Antibody (CST #12503) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Caldesmon-1 (D5C8D) Rabbit Monoclonal Antibody recognizes endogenous levels of total caldesmon-1 protein. Based on sequence homology, this antibody is expected to cross-react with both the smooth muscle and nonmuscle isoforms. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human caldesmon-1 protein. Background Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5). Alternate Names CAD; CALD1; Caldesmon; caldesmon 1; CDM; H-CAD; HCAD; L-CAD; LCAD; MGC21352; NAG22; testis secretory sperm-binding protein Li 227n Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70-80 nonmuscle, 120-150 smooth muscle Source/Isotype: Rabbit IgG