{"product_id":"cst-12530s","title":"CST,  12530S, APC3 (D3I1V) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying APC3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, APC3 (D3I1V) Rabbit Monoclonal Antibody (CST #12530) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAPC3 (D3I1V) Rabbit Monoclonal Antibody recognizes endogenous levels of total APC3 protein. This antibody does not cross-react with either APC8\/CDC23 or APC6\/CDC16.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human APC3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex\/cyclosome (APC\/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC\/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC\/C subunits have been identified. Like all E3 enzymes, APC\/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed, APC\/C has been shown to interact with UBE2S and UBE2C E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). APC\/C activity is also strictly dependent upon its association with multiple cofactors. For example, the related proteins, Cdc20 and Cdh1\/FZR1, participate in the recognition of APC\/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8). Anaphase-promoting complex subunit 3 (APC3), APC8, and APC6 are components of the tetratricopeptide (TPR) APC\/C subcomplex (9). The presence of APC3 is required for binding of Cdh1\/FZR1 to the APC\/C. This suggests that APC\/C is activated by an association between Cdh1\/FZR1 with APC3 that enables APC\/C to recognize the D-box of substrates (6,10). APC3 localizes to the centrosome and the mitotic spindle, suggesting that APC3 plays a critical role in the transition from metaphase to anaphase (11). Phosphorylation of APC3 at multiple sites during mitosis likely leads to structural changes within the APC\/C by altering subunit interactions or changing affinity for molecules that transiently associate with the APC\/C, such as Cdh1\/FZR1 (12,13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nANAPC3; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; CDC27; CDC27 homolog; CDC27Hs; cell division cycle 27; cell division cycle 27 homolog; cell division cycle 27 homolog (S. cerevisiae); cell division cycle protein 27; Cell division cycle protein 27 homolog; D0S1430E; D0S1430E, D17S978E; D17S978E; H-NUC; HNUC; NUC2; nuc2 homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 97\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797573816489,"sku":"12530S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12530s","provider":"Iright","version":"1.0","type":"link"}