CST, 12536S, EVL Antibody

Polyclonal Antibody for studying EVL. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, EVL Antibody (CST #12536) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity EVL Antibody recognizes endogenous levels of total EVL protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser362 of human EVL protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Ena/VASP-like (EVL) protein is a member of the Ena/VASP family and is involved in actin-associated cytoskeleton remodeling and cell polarity activities including axon guidance and lamellipodia formation in migrating cells (1,2,3). The EVL protein sequence contains an N-terminal EVH1 domain, a Pro-rich SH3 binding domain, and a C-terminal EVH2 domain. EVL domain interactions with G- and F-actin mediates actin nucleation and polymerization (4). Research studies have shown that EVL also regulates DNA repair by direct interaction with RAD51 (5). EVL may function in the DSB repair pathway through the EVH2 domain, which possesses DNA-binding and RAD51 binding activity, thereby coordinating homologous DNA recombination (6,7). Research studies have shown EVL expression is up-regulated in human breast cancer associated with clinical stages and may be implicated in invasion and/or metastasis of human breast cancer (8). Alternate Names Ena/vasodilator-stimulated phosphoprotein-like; Ena/VASP-like protein; Enah/Vasp-like; epididymis secretory sperm binding protein; EVL; RNB6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 52 SOURCE: Rabbit