{"product_id":"cst-12538s","title":"CST,  12538S, SAM68 Antibody","description":"Polyclonal Antibody for studying Sam68. Validated for Western Blotting. Highly specific and rigorously validated in-house, SAM68 Antibody (CST #12538) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSAM68 Antibody recognizes endogenous levels of total SAM68 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SAM68 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nSrc associated in mitosis 68 kDa (SAM68) is a member of the signal transduction and activation of RNA (STAR) family of RNA binding proteins (1,2). SAM68 is a multi-functional protein with roles in signal transduction, cell cycle regulation, transcription, and alternative splicing (1-3). SAM68 acts as a scaffold protein mediating responses to various stimuli that activate receptors, such as the T-cell receptor and the insulin receptor, linking activation of various signal transduction pathways with post-transcriptional gene regulation (3). SAM68 is a target for phosphorylation by Src-related kinases and Erk1\/2 mitogen-activated protein kinases. Phosphorylation of SAM68 regulates both its affinity for RNA and alternative splicing of RNAs like CD44 receptor mRNA (1,4). Many of the activities of SAM68 and its targets for alternative splicing have been implicated in the development and progression of spinal muscular atrophy and cancers such as prostate and breast carcinomas, where SAM68 is frequently upregulated (1,5-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ34027; GAP-associated tyrosine phosphoprotein p62; GAP-associated tyrosine phosphoprotein p62 (Sam68); KH domain containing, RNA binding, signal transduction associated 1; KH domain-containing, RNA-binding, signal transduction-associated protein 1; KH RNA binding domain containing, signal transduction associated 1; KHDR1; KHDRBS1; p21 Ras GTPase-activating protein-associated p62; p62; p68; SAM68; Src-associated in mitosis 68 kDa protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 68\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797574013097,"sku":"12538S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12538s","provider":"Iright","version":"1.0","type":"link"}