CST, 12565S, Exportin 5 (D7W6W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Exportin-5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Exportin 5 (D7W6W) Rabbit Monoclonal Antibody (CST #12565) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Exportin 5 (D7W6W) Rabbit Monoclonal Antibody recognizes endogenous levels of total exportin 5 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human exportin 5 protein. Background Import and export through the nuclear envelope (NE) via facilitated translocation is important for many cellular processes including protein synthesis and miRNA biogenesis (1). Exportin 5 (XPO5) is a member of the importin β family of proteins (2) and functions in tRNA export in a sequence dependent fashion. More recently, it has been shown to export pre-miRNA by a RanGTPase-driven process from the nucleus to the cytoplasm, where pre-miRNA processing occurs to produce mature miRNAs (1,3). Study of the miRNA biosynthesis pathway is essential toward understanding the process of oncogenesis as global downregulation of miRNAs and the resulting alterations in expression of tumor suppressor and oncogenic proteins is a common phenotype of cancers cells (3,4). Research studies have shown that disruption of exportin 5 functions in many types of cancers including breast and lung, where pre-miRNA accumulates in the nucleus and miRNA maturation is impaired (2,3). Alternate Names Exp5; exportin 5; Exportin-5; FLJ14239; FLJ32057; FLJ45606; KIAA1291; Ran-binding protein 21; RANBP21; XPO5 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 136 Source/Isotype: Rabbit IgG