{"product_id":"cst-12576t","title":"CST,  12576T, Cleaved Histone H3 (Thr22) (D7J2K) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying H3 (Thr22) cleaved. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Histone H3 (Thr22) (D7J2K) Rabbit Monoclonal Antibody (CST #12576) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Histone H3 (Thr22) (D7J2K) Rabbit Monoclonal Antibody recognizes endogenous levels of histone H3 protein when cleaved at Thr22. This antibody shows a preference for histone H3 protein when cleaved at Thr22, but also recognizes full length histone H3.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr22 of human histone H3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nModulation of chromatin structure has a critical role in the control of various DNA directed activities such as transcription, DNA replication, and repair (1). The basic unit of chromatin, the nucleosome, consists of two turns of DNA wrapped around two copies each of four core histone proteins (H2A, H2B, H3, and H4) (2,3). Amino-terminal tails of histones undergo various post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination in response to physiological and environmental stimuli. These modifications modulate the accessibility of chromatin to effector proteins as well as act as binding sites for specific histone modification recognizing effector proteins that regulate gene expression (1,4,5). Such alterations in chromatin modifications and architecture that accompany gene expression changes have been observed during embryonic stem cell differentiation (6). One of the ways in which chromatin modifications may be altered in stem cells involves regulated proteolysis of histone H3 by Cathepsin L. Cathepsin L cleaves the histone H3 amino-terminal tail predominantly at Thr22 in differentiating stem cells, leading to removal of histone modification marks which could then influence the expression patterns of developmentally regulated genes (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nH3; H3 clustered histone 1; H3 histone family, member A; H3\/A; H31; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3FB; H3FC; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; Histone H3; Histone H3.1; Histone H3\/a; Histone H3\/b; Histone H3\/c; Histone H3\/d; Histone H3\/f; Histone H3\/h; Histone H3\/i; Histone H3\/j; Histone H3\/k; Histone H3\/l\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 15\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797574963369,"sku":"12576T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12576t","provider":"Iright","version":"1.0","type":"link"}