{"product_id":"cst-12582t","title":"CST,  12582T, ELAVL1\/HuR (D9W7E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ELAVL1. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, ELAVL1\/HuR (D9W7E) Rabbit Monoclonal Antibody (CST #12582) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:50\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 66006 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nELAVL1\/HuR (D9W7E) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELAVL1\/HuR protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His201 of human ELAVL1\/HuR protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins includes ELAVL1\/HuR, ELAVL2\/HuB, ELAVL3\/HuC and ELAVL4\/HuD (1). ELAVL1\/HuR is ubiquitously expressed whereas expression of the other three members is neuronal-specific (1). ELAVL\/Hu proteins are highly conserved RNA-binding proteins (1). Besides three RNA recognition motifs, these proteins also contain nuclear localization signals that enable them to shuttle between nucleus and cytoplasm (2). Upon inhibition of transcription by actinomycin D, ELAVL1\/HuR relocates from nucleus to cytoplasm where it binds the AU-rich elements within 3' UTRs to stabilize mRNAs (3, 4). ELAVL1\/HuR is suggested to increase translation by binding to mRNAs (5,6). In addition, ELAVL1\/HuR interacts with microRNAs (miRNAs) (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV like RNA binding protein 1; ELAV-like protein 1; ELAV1; ELAVL1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; Hu-antigen R; Hua; HUR; MelG\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797575258281,"sku":"12582T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12582t","provider":"Iright","version":"1.0","type":"link"}