CST, 12647S, Lefty1 (D7E3G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LEFTY1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Lefty1 (D7E3G) Rabbit Monoclonal Antibody (CST #12647) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 25374 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Lefty1 (D7E3G) Rabbit Monoclonal Antibody recognizes transfected levels of total lefty1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro108 of human lefty1 protein. Background Lefty proteins are members of the TGF-β family of cell signaling molecules that are involved in growth and development (1,2). Named for their role in left-right axis determination and their exclusive expression on the left side of the developing mouse embryo, lefty1 and lefty2 contain a cysteine-knot motif that is characteristic of TGF-β related proteins, but lack an alpha-helix and a cysteine residue critical for ligand dimerization (3). Early in vertebrate embryogenesis, lefty represses TGF-β signaling by inhibiting the phosphorylation of Smad2 following activation of the TGF-β receptor (4). Down-regulated very early upon differentiation (5), lefty proteins act as extracellular antagonists of the signaling pathway for Nodal, a TGF-β ligand critical for left-right patterning and formation of the mesoderm and endoderm (6). Similar to other members of the TGF-β superfamily, lefty proproteins undergo cleavage to release a bioactive protein (7). The biologically active 42 kDa lefty precursor and the 28 kDa polypeptide have been shown to induce MAPK activity (7). Alternate Names BMP-17; BMP17; Left-right determination factor 1; Left-right determination factor B; LEFTB; lefty b; lefty-1; lefty-b; lefty1; leftyb; LFTY1; Protein lefty-1; Protein lefty-B; stra3 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 40 Source/Isotype: Rabbit IgG