CST, 12655T, THOC4/ALY (D3R4R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying THOC4. Validated for WB,IP,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, THOC4/ALY (D3R4R) Rabbit Monoclonal Antibody (CST #12655) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity THOC4/ALY (D3R4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total THOC4/ALY protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala192 of human THOC4/ALY protein. Background mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing and packaging of mRNAs in the form of an mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY, via its interaction with the splicing factor UAP56, forming a large complex termed the transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus and into the cytoplasm (1-3,6). Alternate Names Ally of AML-1 and LEF-1; ALY; ALY/REF; Aly/REF export factor; ALYREF; BEF; bZIP enhancing factor; bZIP-enhancing factor BEF; REF; THO complex 4; THO complex subunit 4; Tho4; THOC4; Transcriptional coactivator Aly/REF Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG