{"product_id":"cst-12655t","title":"CST,  12655T, THOC4\/ALY (D3R4R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying THOC4. Validated for WB,IP,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, THOC4\/ALY (D3R4R) Rabbit Monoclonal Antibody (CST #12655) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor more information about the RBP-eCLIP service please visit Eclipsebio .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:400\neCLIP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTHOC4\/ALY (D3R4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total THOC4\/ALY protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala192 of human THOC4\/ALY protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nmRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing and packaging of mRNAs in the form of an mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4\/ALY, via its interaction with the splicing factor UAP56, forming a large complex termed the transcription-export complex (TREX) (1,2,5). THOC4\/ALY then directly interacts with NXF1\/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus and into the cytoplasm (1-3,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAlly of AML-1 and LEF-1; ALY; ALY\/REF; Aly\/REF export factor; ALYREF; BEF; bZIP enhancing factor; bZIP-enhancing factor BEF; REF; THO complex 4; THO complex subunit 4; Tho4; THOC4; Transcriptional coactivator Aly\/REF\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 27\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797576110249,"sku":"12655T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12655t","provider":"Iright","version":"1.0","type":"link"}