CST, 12661S, IL-17RA (D1Y4C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL17R. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-17RA (D1Y4C) Rabbit Monoclonal Antibody (CST #12661) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IL-17RA (D1Y4C) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-17RA protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant fragment of the human IL-17RA extracellular domain. Background The IL-17 family of cytokines consists of IL-17A-F, and their receptors include IL-17RA-RE (1). IL-17 cytokines are produced by a variety of cell types including the Th17 subset of CD4+ T cells, as well as subsets of γδ T cells, NK cells, and NKT cells (2). IL-17A and IL-17F, the most well-studied of the IL-17 cytokines, contribute to fungal and bacterial immunity by inducing expression of proinflammatory cytokines, chemokines, and antimicrobial peptides (2). In addition, IL-17A contributes to the pathogenesis of several autoimmune diseases (3). IL-17E promotes Th2 cell responses (4). The roles of IL-17B, IL-17C, and IL-17D are less clear, however these family members also appear to have the capacity to induce proinflammatory cytokines (1,5,6). IL-17 receptors have an extracellular domain, a transmembrane domain, and a SEFIR domain. They are believed to signal as homodimers, heterodimers, or multimers through their SEFIR domain by recruiting the SEFIR domain-containing adaptor Act1 (7). Unlike most cytokines that signal through Jak/STAT pathways, IL-17 signaling results in NF-κB activation (8). IL-17RA associates with IL-17RC to mediate signaling by homodimers and heterodimers of IL-17A and IL-17F (9,10). IL-17RA is broadly expressed with highest expression in hematopoietic cells (11). Alternate Names CANDF5; CD217; CDw217; hIL-17R; I17RA; IL-17 receptor A; IL-17RA; IL17R; IL17RA; IMD51; interleukin 17 receptor A; Interleukin-17 receptor A; MGC10262 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120-170 Source/Isotype: Rabbit IgG