CST, 12668T, Reptin/RuvBL2 (D8N3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Reptin/RUVBL2. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Reptin/RuvBL2 (D8N3J) Rabbit Monoclonal Antibody (CST #12668) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Reptin/RuvBL2 (D8N3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total Reptin/RuvBL2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly263 of human Reptin/RuvBL2 protein. Background Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the AAA+ (ATPase associated with diverse cellular activities) superfamily of proteins, and are putatively homologous to bacterial RuvB proteins that drive branch migration of Holliday junctions (1). Reptin and Pontin function together as essential components of chromatin remodeling and modification complexes, such as INO80, TIP60, SRCAP, and Uri1, which play key roles in regulating gene transcription (1,2). In their capacity as essential transcriptional co-regulators, Reptin and Pontin have both been implicated in oncogenic transformations, including those driven by c-Myc, β-catenin, and E1A (2-7). Reptin plays a role in modulating cellular responses to hypoxia. Hypoxia induced methylation of Reptin by the methyltransferase G9a leads to its recruitment to hypoxia responsive promoters, where it negatively regulates transcription of these genes (8). In addition to transcriptional regulatory roles, Reptin also participates in the telomerase biogenesis processes as part of the telomerase complex. Reptin is involved in DNA damage response as part of the TIP60 acetyltransferase complex that stimulates ATM kinase activity necessary for phosphorylation of proteins involved in both checkpoint activation and DNA repair (9,10). Alternate Names 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; CGI-46; ECP-51; ECP51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; INO80J; Repressing pontin 52; REPTIN; Reptin 52; Reptin52; reptin52 protein; RuvB (E coli homolog)-like 2; RuvB like AAA ATPase 2; RuvB-like 2; RuvB-like 2 (E. coli); RUVB2; RUVBL2; RVB2; TAP54-beta; TBP-interacting protein, 48-KD; TIH2; TIP48; TIP49B; TIP60-associated protein 54-beta Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG