CST, 12684S, AFAP1L2/XB130 (D1B5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying AFAP1L2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, AFAP1L2/XB130 (D1B5) Rabbit Monoclonal Antibody (CST #12684) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity AFAP1L2/XB130 (D1B5) Rabbit Monoclonal Antibody recognizes endogenous levels of total AFAP1L2/XB130 protein. In some cell extracts this antibody recognizes an 80 kDa background protein of unknown origin. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly638 of human AFAP1L2/XB130 protein. Background The actin-filament associated protein (AFAP) family consists of AFAP1, AFAP1L1, and AFAP1L2/XB130, a group of structurally similar proteins that play distinct roles in the regulation of cytoskeletal dynamics and signal transduction. Actin filament-associated protein 1-like 2 (AFAP1L2, XB130) is an adaptor protein that regulates signaling downstream of multiple kinases, including Src, Akt, and the thyroid specific kinase RET/PTC (1-3). Through these pathways, AFAP1L2/XB130 mediates transcriptional regulation, cell proliferation, motility, and microRNA expression (4,5). Research has shown that AFAP1L2/XB130 is involved in the proliferation and survival of thyroid tumor cells (6), and may have value in gastric cancer prognosis (7). Alternate Names actin filament associated protein 1 like 2; actin filament associated protein 1-like 2; Actin filament-associated protein 1-like 2; AF1L2; AFAP1-like protein 2; AFAP1L2; CTB-1144G6.4; CTB-1144G6.6; FLJ14564; KIAA1914; XB130 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG