{"product_id":"cst-12703t","title":"CST,  12703T, IL-1 beta (D3U3E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IL1 beta. Validated for WB,WB,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-1 beta (D3U3E) Rabbit Monoclonal Antibody (CST #12703) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 18689 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIL-1 beta (D3U3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-1β protein. This antibody is not observed to detect endogenous levels of mature IL-1β. This antibody can detect up to 100 pg of recombinant mature IL-1β.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant human IL-1β protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCatabolin; IL-1; IL-1 beta; IL1-BETA; IL1B; IL1beta; IL1F2; interleukin 1 beta; interleukin 1, beta; interleukin 1beta; Interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 17, 31\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797577322665,"sku":"12703T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12703t","provider":"Iright","version":"1.0","type":"link"}