CST, 12712S, Phospho-TPOR (Tyr626) (D3H7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TpoR (Tyr626) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-TPOR (Tyr626) (D3H7B) Rabbit Monoclonal Antibody (CST #12712) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-TPOR (Tyr626) (D3H7B) Rabbit Monoclonal Antibody recognizes endogenous levels of TPOR protein only when phosphorylated at Tyr626. This antibody cross-reacts with a 30 kDa protein of unknown origin. This antibody may cross-react with some tyrosine-phosphorylated proteins including the EGF Receptor, ROS1, and FLT3. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr626 of human TPOR protein. Background Thrombopoietin receptor (TPOR, c-Mpl) is a hematopoietic receptor that binds the growth factor, thrombopoietin (TPO), responsible for regulation of platelet production (1-3). Expression of TPOR by megakaryocytes is required for megakaryocyte growth and development (4). TPOR is also expressed by hematopoietic stem cells and is required for stem cell maintenance and expansion (5). Studies show that mice lacking either TPOR or TPO have severely reduced numbers of platelets and megakaryocytes as well as decreased numbers of other hematopoietic lineages (4,5). Binding of TPO to TPOR induces receptor dimerization that leads to phosphorylation and activation of the tyrosine kinase Jak2. Activated Jak2 associates with the cytoplasmic domain of TPOR (6,7) and phosphorylates TPOR at Tyr626 and Tyr631 (8). These phosphorylated tyrosine residues provide docking sites for downstream signaling molecules including Stat3, Stat5, Shc, and SHIP (7-9). Alternate Names C-MPL; CD110; MPL; MPL proto-oncogene, thrombopoietin receptor; MPLV; Myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; Proto-oncogene c-Mpl; THCYT2; THPOR; Thrombopoietin receptor; TPO-R; TPOR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 85-90 Source/Isotype: Rabbit IgG