CST, 12713S, uPAR (D7X2N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying uPAR. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, uPAR (D7X2N) Rabbit Monoclonal Antibody (CST #12713) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 45547 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity uPAR (D7X2N) Rabbit Monoclonal Antibody recognizes endogenous levels of total uPAR protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg303 of human uPAR protein. Background The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65 kDa, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35 kDa) (1-3). It is a central player in the plasminogen activation pathway. uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4). Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5). Therefore, uPAR plays a key role in regulating extracellular proteolysis. In addition, uPAR plays an important role in regulating cell proliferation, adhesion and mobility (6,7). Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9). Alternate Names CD87; MO3; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; U-PAR; u-plasminogen activator receptor form 2; UPAR; URKR; Urokinase plasminogen activator surface receptor; urokinase-type plasminogen activator (uPA) receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35-65 Source/Isotype: Rabbit IgG