CST, 12717T, Toll-like Receptor 6 (D1Z8B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TLR6. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Toll-like Receptor 6 (D1Z8B) Rabbit Monoclonal Antibody (CST #12717) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Toll-like Receptor 6 (D1Z8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total TLR6 protein. This antibody cross-reacts with a 72 kDa protein of unknown origin. This antibody is not approved for IP in mouse samples. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro47 of human and mouse TLR6 protein. Background Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in , play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like/TIR-associated protein (MAL/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. Toll-like receptor 6 (TLR6) heterodimerizes with TLR2 and is expressed on the cell surface where it recognizes fungal zymosan and bacterial lipoproteins (15). In addition, a heterodimer of TLR4 and TLR6 was recently shown to assemble downstream of CD36 signaling and contribute to sterile inflammation in response to CD36 ligands, including low-density lipoprotein and β-amyloid (16). Alternate Names CD286; TLR6; toll like receptor 6; Toll-like receptor 6 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 90-110 Source/Isotype: Rabbit IgG