{"product_id":"cst-12721l","title":"CST,  12721L, NRF2 (D1Z9C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NRF2. Validated for WB,WB,IP,IF,F,ChIP,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, NRF2 (D1Z9C) Rabbit Monoclonal Antibody (CST #12721) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:1600 - 1:6400\nChromatin IP: 1:200\nChromatin IP-seq: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 84599 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNRF2 (D1Z9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total NRF2 protein.\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala275 of human NRF2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintain cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHEBP1; IMDDHH; NF-E2-related factor 2; NF2L2; NFE2-related factor 2; NFE2L2; Nrf-2; NRF2; nuclear factor (erythroid-derived 2)-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2 like 2; Nuclear factor, erythroid derived 2, like 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nMW (kDa): 97-100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797578109097,"sku":"12721L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12721l","provider":"Iright","version":"1.0","type":"link"}