CST, 12782S, XBP-1s (D2C1F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying XBP1S/XBP1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, XBP-1s (D2C1F) Rabbit Monoclonal Antibody (CST #12782) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity XBP-1s (D2C1F) Rabbit Monoclonal Antibody recognizes endogenous levels of the total spliced isoform of XBP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu64 of human XBP1 protein. Background Following protein synthesis, secretory, intra-organellar, and transmembrane proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. The accumulation of unfolded proteins within the ER triggers an adaptive mechanism known as the unfolded protein response (UPR) that counteracts compromised protein folding (1). The transmembrane serine/threonine kinase IRE1, originally identified in , is a proximal sensor for the UPR that transmits the unfolded protein signal across the ER membrane (2-4). The human homolog IRE1α was later identified and is ubiquitously expressed in human tissues (5). Upon activation of the unfolded protein response, IRE1α splices X-box binding protein 1 (XBP-1) mRNA through an unconventional mechanism using its endoribonuclease activity (6). This reaction converts XBP-1 from an unspliced XBP-1u isoform to the spliced XBP-1s isoform, which is a potent transcriptional activator that induces expression of many UPR responsive genes (6). Alternate Names Tax-responsive element-binding protein 5; TREB-5; TREB5; X-box binding protein 1; X-box-binding protein 1; X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form; XBP-1; XBP-1S; XBP1; XBP1 iso2; XBP1(S); XBP2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG