{"product_id":"cst-12793s","title":"CST,  12793S, Glutamate Dehydrogenase 1\/2 (D9F7P) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GLUD1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Glutamate Dehydrogenase 1\/2 (D9F7P) Rabbit Monoclonal Antibody (CST #12793) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:800 - 1:3200\nImmunofluorescence (Immunocytochemistry): 1:600 - 1:1200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 80063 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGlutamate Dehydrogenase 1\/2 (D9F7P) Rabbit Monoclonal Antibody recognizes endogenous levels of total glutamate dehydrogenase 1 and 2 proteins. Species cross-reactivity for IHC-P and IF-IC is in human only.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro190 of human glutamate dehydrogenase 1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGlutamate dehydrogenase is a mitochondrial enzyme that catalyzes the oxidative deamination of glutamate to α-ketoglutarate through association with the cofactor nicotinamide adenine dinucleotide phosphate (1). Glutamate dehydrogenase is highly expressed in various tissues such as the liver, brain, kidney, heart, pancreas, ovaries, and testis. Two isoforms produced by two distinct genes are found in mammalian tissues. The gene is ubiquitously expressed (2), while the gene is specifically expressed in testicular tissues and astrocytes (3,4). Glutamate dehydrogenase links glutamate to the Krebs cycle, thereby playing a critical role in the regulation of energy homeostasis. Research studies have shown that changes in glutamate dehydrogenase activity in pancreatic β-cells can cause a hyperinsulinism syndrome (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDHE3; epididymis secretory sperm binding protein; epididymis tissue sperm binding protein Li 18mP; GDH; GDH 1; GDH1; GLUD; GLUD1; glutamate dehydrogenase (NAD(P)+); glutamate dehydrogenase 1; Glutamate dehydrogenase 1, mitochondrial; MGC132003\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 52\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797580894377,"sku":"12793S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12793s","provider":"Iright","version":"1.0","type":"link"}