CST, 12800T, STF-1 (D1Z2A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STF-1. Validated for WB,IP,IF,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, STF-1 (D1Z2A) Rabbit Monoclonal Antibody (CST #12800) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq Specificity / Sensitivity STF-1 (D1Z2A) Rabbit Monoclonal Antibody recognizes endogenous levels of total STF-1 protein. This antibody does not cross-react with LRH-1/NR5A2. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu184 of human STF-1 protein. Background The orphan nuclear receptor, steroidogenic factor 1 (STF-1, also called Ad4BP), is encoded by the gene and plays an instrumental role in directing the transcriptional control of steroidogenesis (1). Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global (2) and tissue-specific knockout mice (3-6) have demonstrated that STF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency (7,8). Furthermore, dysregulation of STF-1 has been linked to diseases such as endometriosis (9) and adrenocortical carcinoma (10). Like other nuclear hormone receptors, STF-1 has a modular domain structure composed of an amino-terminal zinc finger DNA-binding domain, a ligand-binding domain, a carboxy-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. STF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain (11). STF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors (12). In addition to phosphorylation at Ser203, STF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses STF-1 function (13,14), acetylation enhances its transcriptional activity (15). Alternate Names AD4BP; adrenal 4 binding protein; Adrenal 4-binding protein; ELP; FTZ1; FTZF1; Fushi tarazu factor homolog 1; hSF-1; NR5A1; nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1; nuclear receptor subfamily 5, group A, member 1; POF7; SF-1; SF1; SPGF8; SRXX4; SRXY3; Steroid hormone receptor Ad4BP; Steroidogenic factor 1; steroidogenic factor 1 nuclear receptor; steroidogenic factor-1; STF-1; STF1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG